Gel Electrophoresis Buffers and Diluents
Electron Microscopy Sciences Clear-Mount with PIPE Buffer 100 ML
Clear-Mount is a water based mounting medium designed for the permanent mounting of hydrated tissues, which may be damaged with organic solvents. Such samples include cell smears, with peroxidase and alkaline phosphatase chromogens.
This mounting medium preserves Fast Red, Aminoethylycarbazole (AEC), NBT/BCIP, INT/BCIP chromogens and is also compatible with counter-stain such as Hematoxylin and Nuclear Fast Red. It is also suitable for chromogens like DAB and DAB with Nickel and Cobalt. It is not compatible with H and E staining.
Application: Mounting of Immunohisto slides.
Waters Corp Pico-Tag Diluent
The Waters Pico Tag Method has been widely used for amino acid analysis for over two decades PicoTag Diluent, used in the Waters Pico Tag Method, is sold separetly. This diluent is in a 100mL Bottle.
Zymo Research Corporation M-Elution Buffer, 1 ml
M-Elution Buffer (1.0 ml) for use with EZ DNA Methylation™ bisulfite conversion Kits. Sold individually or as part of a bisulfite conversion kit.
Zymo Research Corporation ZymoBIOMICS™ DNA Binding Buffer, 100 mL
It is certified to be low-bioburden to ensure minimal background contaminants for low abundance microbiome samples.
Boston Bioproducts Inc Laemmli SDS-Sample Buffer (6X, Reducing) - 25ml
The 6X laemmli sample buffer is a critical component in the preparation of protein samples for SDS-PAGE, ensuring the resolution of protein bands. Its composition typically includes Tris-HCl, glycerol, SDS, and a tracking dye like Bromophenol Blue. In the reducing Laemmli sample buffer, a reducing agent such as β-mercaptoethanol or dithiothreitol (DTT) is added to break disulfide bonds within protein subunits, allowing them to separate by size in electrophoresis.
Vector Laboratories BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit 200 ml
BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit, 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium, forms an indigo, heat-stable reaction product that can be dehydrated, cleared, and permanently mounted. This substrate can also be used for developing blots or for in situ hybridization. With 20-30 minute development times, the sensitivity of this substrate is equivalent to the other alkaline phosphatase substrates. However, BCIP/NBT will continue to develop from several hours to overnight making it one of the most sensitive chromogenic substrates. The stock solutions in this kit are provided in convenient dropper bottles, in sufficient volume for 200 ml working solution.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffer solution, 100mL
Phosphate buffer solution has been used in the inhibition assay of α-glucosidase activity, for measuring calcium deposition, and in trypsin grafting.
Aqua Solutions Acetate Buffer pH 4.2 - 4.6 APHA For Iron Analysis (1L)
Acetate Buffer pH 4.2 - 4.6 APHA For Iron Analysis (1L)
Boston Bioproducts Inc Transfer Buffer (10X, Electro Blotting) - 4L
The 10X Transfer Buffer-Electro Blotting is the most commonly used buffer in the wet transfer system for the electrotransfer of proteins from SDS-PAGE gels to nitrocellulose or PVDF membranes. The addition of 10% methanol in thhis buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.
Research Products International Corp Dry Powder Milk, 100 Grams
Nonfat dry milk for use as a blocking agent during Western Blotting to block non-specific antibody binding and reduce background signal.
